1HK4
HUMAN SERUM ALBUMIN COMPLEXED WITH THYROXINE (3,3',5,5'-TETRAIODO-L-THYRONINE) and myristic acid (tetradecanoic acid)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 1999-01-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.580, 38.910, 95.260 |
Unit cell angles | 90.00, 105.43, 90.00 |
Refinement procedure
Resolution | 37.500 * - 2.400 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e7g |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.180 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.500 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.079 | 0.221 |
Number of reflections | 24201 | |
<I/σ(I)> | 5.6 | 2.7 |
Completeness [%] | 90.6 | 76 |
Redundancy | 2.1 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 20 (mM) | pH7.0-7.5 |