1HJZ
Crystal structure of AF1521 protein containing a macroH2A domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.930, 55.720, 62.740 |
| Unit cell angles | 90.00, 115.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.100 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 * | 0.151 |
| Number of reflections | 40692 | |
| <I/σ(I)> | 19.1 | 6.7 |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 3.4 * | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 17 * | 0.1M MES BUFFER, PH 6.5 18% PEG 5000 MME, 0.2M AMMONIUM SULPHATE, 25MM MANGANESE CHLORIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 18 (%(w/v)) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | 25 (mM) | ||
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | drop | dithiothreitol | 5 (mM) |
