1HJZ

Crystal structure of AF1521 protein containing a macroH2A domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	119.930, 55.720, 62.740
Unit cell angles	90.00, 115.15, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.203
Rwork	0.203
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.185
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.100	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.047 *	0.151
Number of reflections	40692
<I/σ(I)>	19.1	6.7
Completeness [%]	98.5	98.5
Redundancy	3.4 *	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	17 *	0.1M MES BUFFER, PH 6.5 18% PEG 5000 MME, 0.2M AMMONIUM SULPHATE, 25MM MANGANESE CHLORIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	18 (%(w/v))
2	1	reservoir	MES	0.1 (M)	pH6.5
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir		25 (mM)
5	1	drop	protein	10 (mg/ml)
6	1	drop	dithiothreitol	5 (mM)