1HJL
Biochemical and Structural Analysis of the Molybdenum Cofactor Biosynthesis protein MobA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.224, 41.752, 54.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.230 - 2.000 |
| Rwork | 0.171 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e5k |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.851 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.163 |
| Number of reflections | 12166 | |
| <I/σ(I)> | 17.9 | 4.4 |
| Completeness [%] | 98.1 | 86.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Stevenson, C.E., (2000) Structure, 8, 1115. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 20 (%(v/v)) | |
| 3 | 1 | reservoir | PEG1500 | 2 (%(w/v)) | |
| 4 | 1 | reservoir | citric acid | 100 (mM) |
