1HJK

ALKALINE PHOSPHATASE MUTANT H331Q

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1995-11-01
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	I 2 2 2
Unit cell lengths	195.520, 167.860, 77.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.177
Rwork	0.177
R-free	0.20000
RMSD bond length	0.018
RMSD bond angle	24.800 *
Data reduction software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	80.000
High resolution limit [Å]	2.300
Rmerge	0.090
Total number of observations	120731 *
Number of reflections	51270
Completeness [%]	90.0
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5 *		55% SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2, 10 MM ZNCL2, 2 MM NAH2PO4 AT PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)