1HIW
TRIMERIC HIV-1 MATRIX PROTEIN
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.423, 91.163, 73.997 |
| Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.33200 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.195 |
| Total number of observations | 104117 * | |
| Number of reflections | 36171 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 98.4 | 81.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | EGTA | 1 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 5 (mM) | |
| 5 | 1 | drop | reservoir solution | ||
| 6 | 1 | reservoir | PEG2000 | 30 (%(w/w)) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 8 | 1 | reservoir | 0.1 (M) |
