1HI8

RNA dependent RNA polymerase from dsRNA bacteriophage phi6

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-15
Detector	SBC
Wavelength(s)	0.97923, 0.97939, 0.99988, 0.92873
Spacegroup name	P 32
Unit cell lengths	110.000, 110.000, 159.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.28
Rwork	0.280
R-free	0.31600
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	2.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.240
Total number of observations	430000 *
Number of reflections	66000
<I/σ(I)>	17.2	1.4
Completeness [%]	88.0	66 *
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.7	293 *	Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, 56, 1473. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	MES	0.1 (M)