1HH5

cytochrome c7 from Desulfuromonas acetoxidans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1997-10-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	37.190, 43.830, 44.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.000 - 1.900
R-factor	0.194
Rwork	0.194
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1new
RMSD bond length	0.008
RMSD bond angle	26.300 *
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.082 *	0.182 *
Number of reflections	5213
<I/σ(I)>	5.6	2.1
Completeness [%]	92.7	90 *
Redundancy	3.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6		20-22% PEG 1000, 0.1 M TRIS PH 7.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	Tris	20 (mM)
3	1	reservoir	PEG1000	20-22 (%)
4	1	reservoir	Tris	0.1 (M)