1HGX
HYPOXANTHINE-GUANINE-XANTHINE PHOSPHORIBOSYLTRANSFERASE (HGXPRTASE)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1995-08-16 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.960, 74.920, 55.720 |
Unit cell angles | 90.00, 110.77, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HUMAN HGPRTASE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.420 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | |
High resolution limit [Å] | 1.900 * | 1.900 * |
Rmerge | 0.063 | 0.380 * |
Total number of observations | 97259 * | |
Number of reflections | 27314 | |
Completeness [%] | 91.2 | 55.6 * |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium salfate | 0.2 (M) | |
2 | 1 | reservoir | Bis-Tris | 0.1 (M) | |
3 | 1 | reservoir | PEG8000 | 28-32 (%(w/v)) | |
4 | 1 | drop | HGX-PRTase | 10 (mg/ml) | |
5 | 1 | drop | Bis-Tris | 50 (mM) | |
6 | 1 | drop | 6 (mM) |