1HFZ
ALPHA-LACTALBUMIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1994-11-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.87, 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.620, 60.130, 77.180 |
Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.30300 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.730 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.300 * |
Rmerge | 0.078 |
Number of reflections | 18629 |
Completeness [%] | 80.0 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | 16 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | 0.15 (M) | ||
3 | 1 | reservoir | PEG4000 | 20-25 (%) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) |