1HFJ
Asparaginase from Erwinia chrysanthemi, hexagonal form with sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-09-15 |
Detector | R-AXIS II |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 91.400, 91.400, 346.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.16 |
Rwork | 0.160 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FROM REFERENCE 3 |
RMSD bond length | 0.008 |
RMSD bond angle | 23.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.105 * | 0.280 * |
Total number of observations | 89335 * | |
Number of reflections | 29191 | |
<I/σ(I)> | 10 | 2.2 |
Completeness [%] | 84.3 | 66.8 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.8 * | 20 * | Kozak, M., (2000) Acta Biochim. Pol., 47, 807. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | sodium citrate | 10 (mM) | |
3 | 1 | reservoir | MPD | 46-48 (%) | |
4 | 1 | reservoir | sodium citrate | 100 (mM) | |
5 | 1 | reservoir | 10-20 (mM) |