1HFJ

Asparaginase from Erwinia chrysanthemi, hexagonal form with sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-09-15
Detector	R-AXIS II
Spacegroup name	P 61 2 2
Unit cell lengths	91.400, 91.400, 346.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.16
Rwork	0.160
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FROM REFERENCE 3
RMSD bond length	0.008
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.105 *	0.280 *
Total number of observations	89335 *
Number of reflections	29191
<I/σ(I)>	10	2.2
Completeness [%]	84.3	66.8
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.8 *	20 *	Kozak, M., (2000) Acta Biochim. Pol., 47, 807. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	sodium citrate	10 (mM)
3	1	reservoir	MPD	46-48 (%)
4	1	reservoir	sodium citrate	100 (mM)
5	1	reservoir		10-20 (mM)