1HFA

CALM-N N-terminal domain of clathrin assembly lymphoid myeloid leukaemia protein, PI(4,5)P2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	CCD
Collection date	2000-03-23
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	77.725, 77.725, 122.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.940 - 2.000
R-factor	0.19844 *
Rwork	0.197
R-free	0.23012 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hf8
RMSD bond length	0.032 *
RMSD bond angle	2.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.103	1.153
Number of reflections	26075
<I/σ(I)>	14.3	1.5
Completeness [%]	100.0	100
Redundancy	7.1	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		0.1M HEPES, PH 7.5, 12% PEG 8K, 8% ETHYLENE GLYCOL, CRYSTALS SOAKED IN 1MM LIGAND FOR 1 HOUR

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)
2	1	reservoir	PEG8000	12-14 (%)
3	1	reservoir	ethylene glycol	8 (%)