1HDY
THREE-DIMENSIONAL STRUCTURES OF THREE HUMAN ALCOHOL DEHYDROGENASE VARIANTS: CORRELATIONS WITH THEIR FUNCTIONAL DIFFERENCES
Experimental procedure
| Spacegroup name | P 1 |
| Unit cell lengths | 54.360, 44.890, 93.850 |
| Unit cell angles | 92.65, 103.21, 68.77 |
Refinement procedure
| Resolution | 7.000 - 2.500 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.220 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.060 * |
| Total number of observations | 47784 * |
| Number of reflections | 24361 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium phosphate | 50 (mM) | |
| 2 | 1 | reservoir | NAD+ | 5 (mM) | |
| 3 | 1 | reservoir | 4-iodopyrazole | 1 (mM) | |
| 4 | 1 | reservoir | enzyme | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 11.5 (%) |
