1HDR

THE CRYSTALLOGRAPHIC STRUCTURE OF A HUMAN DIHYDROPTERIDINE REDUCTASE NADH BINARY COMPLEX EXPRESSED IN ESCHERICHIA COLI BY A CDNA CONSTRUCTED FROM ITS RAT HOMOLOGUE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	50.650, 138.650, 65.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.169
Rwork	0.169
RMSD bond length	0.016
RMSD bond angle	3.200
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.074 *	0.180 *
Total number of observations	38602 *
Number of reflections	7999 *	1451 *
Completeness [%]	97.0 *
Redundancy	4.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4500
2	1	reservoir	methanol	8 (%)
3	1	reservoir	beta-mercaptoethanol	1 (mM)
4	1	reservoir	Tris phosphate