1HD2
Human peroxiredoxin 5
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.1,0.9175,0.9169,0.855 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.607, 66.607, 123.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.000 - 1.500 |
R-factor | 0.1325 |
R-free | 0.16450 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 0.026 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.000 | 1.720 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.051 | 0.246 |
Total number of observations | 384543 * | |
Number of reflections | 45181 | |
<I/σ(I)> | 24 | 7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.5 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.3 | 18 * | PROTEIN WAS CRYSTALLIZED FROM 1.6 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE BUFFER PH 5.3, 0.2 M POTASSIUM SODIUM TARTRATE, 1 MM DTT, 0.02 % (W/V) SODIUM AZIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | potassium/sodium tartrate | 0.2 (M) | |
5 | 1 | reservoir | 1,4-dithio-DL-threitol | 0.001 (M) | |
6 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |