1HCV
LLAMA HEAVY CHAIN VARIABLE DOMAIN AGAINST ALPHA SUBUNIT OF HCG (HUMAN CHORIONIC GONADOTROPIN)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02-19 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.620, 41.830, 38.520 |
Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.850 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | VH OF FAB FRAGMENT OF ANTIBODY AGAINST 2-PHENYL-OXAZOLONE |
RMSD bond length | 0.009 |
RMSD bond angle | 26.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 1.850 * |
Rmerge | 0.089 * | 0.191 * |
Total number of observations | 92439 * | |
Number of reflections | 8077 * | |
<I/σ(I)> | 9.03 | 3.4 |
Completeness [%] | 99.8 * | 100 * |
Redundancy | 1.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 20 * | VAPOR DIFFUSION, PEG 8K 25%, TRIS 0.1M, PH 8.0, vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 25 (%) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) |