1HCG
STRUCTURE OF HUMAN DES(1-45) FACTOR XA AT 2.2 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 41 21 2 |
Unit cell lengths | 93.660, 93.660, 119.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.200 |
R-factor | 0.168 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.055 |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.200 * | 2.200 * |
Rmerge | 0.087 * | |
Total number of observations | 53755 * | |
Number of reflections | 17121 * | |
Completeness [%] | 61.5 * | 38 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 0.2 (M) | precipitant |
3 | 1 | drop | HEPES | 0.1 (M) | precipitant |
4 | 1 | drop | sodium potassium phosphate | 2.0 (M) | precipitant |
5 | 1 | reservoir | precipitant | 1.9 (M) |