1HBX

Ternary Complex of SAP-1 and SRF with specific SRE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	103
Detector technology	CCD
Collection date	2000-04-15
Detector	ADSC Q4
Spacegroup name	P 21 21 2
Unit cell lengths	142.680, 144.390, 75.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.000 - 3.150
R-factor	0.252
Rwork	0.252
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1srs 1bc7
RMSD bond length	0.008
RMSD bond angle	21.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.042	0.583
Number of reflections	31747
<I/σ(I)>	30.4	1.98
Completeness [%]	98.5	96.7
Redundancy	5.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	PEG 1500, NH4NO3, BIS-TRIS BUFFER, DTT, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)	ternary complex
2	1	drop	PEG1500	8-10 (%)
3	1	drop	Bis-Tris	25 (mM)
4	1	drop		50 (mM)
5	1	drop	dithiothreitol	0.5 (mM)
6	1	reservoir	PEG1500	16-20 (%)
7	1	reservoir	Bis-Tris	50 (mM)
8	1	reservoir		100 (mM)
9	1	reservoir	dithiothreitol	1.0 (mM)