1HBQ

CRYSTAL STRUCTURE OF LIGANDED AND UNLIGANDED FORMS OF BOVINE PLASMA RETINOL-BINDING PROTEIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	46.550, 48.970, 76.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 * - 1.700
R-factor	0.196
Rwork	0.196
RMSD bond length	0.011
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	2.700 *
High resolution limit [Å]	2.200 *
Rmerge	0.059 *
Total number of observations	70544 *
Number of reflections	18382 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	bRBP	6-10 (mg/ml)
2	1	2	sodium chloride	0.1 (M)
3	1	2	cadmium acetate	0.01-1. (mM)
4	1	2	sodium acetate	0.1 (M)