1HB6
Structure of bovine Acyl-CoA binding protein in orthorhombic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-08-15 |
| Detector | MULTIWIRE UCSD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.050, 54.780, 65.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.140 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | UCSD |
| Data scaling software | UCSD |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.076 |
| Number of reflections | 6473 | |
| <I/σ(I)> | 32 | 9.3 |
| Completeness [%] | 94.9 | 91.2 |
| Redundancy | 3.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 15% PEG8000, 5MMCDCL2, 10MM HEPES PH7.5, pH 7.50 |
