1HAI

THE ISOMORPHOUS STRUCTURES OF PRETHROMBIN2, HIRUGEN-AND PPACK-THROMBIN: CHANGES ACCOMPANYING ACTIVATION AND EXOSITE BINDING TO THROMBIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	70.970, 72.500, 72.570
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	7.000 - 2.400
R-factor	0.139
Rwork	0.139
RMSD bond length	0.020
RMSD bond angle	0.050 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.054 *
Number of reflections	13404 *
Completeness [%]	75.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7 *		Bode, W., (1989) EMBO J., 8, 3467. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG6000	16 (%)
2	1	1	phosphate	0.2 (M)