1H9B
ACTIVE MUTANT (Q365->C) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 136.700, 136.700, 121.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.000 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PROTEIN ATOMS OF NADP-BOUND COMPLEX IN THE SAME CRYSTAL FORM 1H9A. |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.730 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.113 * | 0.418 * |
| Total number of observations | 115220 * | |
| Number of reflections | 24865 | 3523 * |
| <I/σ(I)> | 12.7 | 3.7 |
| Completeness [%] | 94.1 | 93 |
| Redundancy | 4.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 * | HANGING DROP VAPOUR DIFFUSION, 2+2 MICROLITER DROPS. THE PROTEIN AT 5MG/ML IN 100MM TRIS-HCL AT PH 7.5 WITH 12.5MM NAD+. DROPS EQUILIBRIATED AGAINST 1.7M UNBUFFERED AMMONIUM SULFATE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | NAD+ | 12.5 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.64 (M) | unbuffered |
