1H8X

Domain-swapped Dimer of a Human Pancreatic Ribonuclease Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-02-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	50.610, 61.430, 75.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.000
R-factor	0.1964
Rwork	0.196
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dza
RMSD bond length	0.006
RMSD bond angle	1.565 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	2.000
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.076	0.315
Total number of observations	56729 *
Number of reflections	16247 *
<I/σ(I)>	7.1	2.4
Completeness [%]	99.4	99.8
Redundancy	3.4 *	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9		pH 9.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ethanol	20 (%(v/v))
3	1	reservoir	Bicine	0.1 (M)	pH9.0