1H8U
Crystal Structure of the Eosinophil Major Basic Protein at 1.8A: An Atypical Lectin with a Paradigm Shift in Specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.328, 57.487, 60.961 |
Unit cell angles | 90.00, 113.20, 90.00 |
Refinement procedure
Resolution | 36.180 - 1.800 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lit |
RMSD bond length | 0.009 |
RMSD bond angle | 24.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 * | 0.229 * |
Total number of observations | 217030 * | |
Number of reflections | 21127 | |
<I/σ(I)> | 16.2 | 5.18 |
Completeness [%] | 96.0 | 91.8 |
Redundancy | 10.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 20 * | 100MM MALONIC ACID PH6, 140MM POTTASIUM DIHYDROGEN PHOSPHATE., pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | reservoir | malonic acid | 100 (mM) | |
3 | 1 | reservoir | potassium phosphate | 160 (mM) | |
4 | 1 | reservoir | L-DTT | 5 (mM) | |
5 | 1 | reservoir | sodium acetate | 160 (mM) |