1H7W

Dihydropyrimidine dehydrogenase (DPD) from pig

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-15
Detector	MARRESEARCH
Wavelength(s)	0.9184, 1.7392, 1.7416
Spacegroup name	P 1 21 1
Unit cell lengths	81.950, 159.290, 163.570
Unit cell angles	90.00, 96.04, 90.00

Refinement procedure

Resolution	19.970 - 1.900
R-factor	0.174
Rwork	0.174
R-free	0.19600
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.066 *	0.240 *
Total number of observations	2358836 *
Number of reflections	326433
<I/σ(I)>	10.9	3.9
Completeness [%]	97.4	91.2
Redundancy	3.3	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7	20 *	100 MM SODIUM CITRATE PH 4.7, 16-20 % POLYETHYLENE GLYCOL 6000, 1 MM DTT

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	19-22 (%(w/v))
2	1	reservoir	sodium citrate	100 (mM)