1H7S
N-terminal 40kDa fragment of human PMS2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9686,0.9788,0.9793,0.98,0.79 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.157, 74.851, 135.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.950 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.062 | 0.318 |
| Number of reflections | 53805 | |
| <I/σ(I)> | 18.5 | 1.6 |
| Completeness [%] | 97.7 | 99.3 |
| Redundancy | 4.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 2-2.4 M NA/K PHOSPHATE (PH 6.2) 0.2 M LICL |
