1H75
Structural basis for the thioredoxin-like activity profile of the glutaredoxin-like protein NrdH-redoxin from Escherichia coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.151, 41.346, 58.668 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 1.700 | |
| Rmerge | 0.058 | |
| Total number of observations | 113655 * | |
| Number of reflections | 8503 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 96.4 | 88.7 * |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.1 | 20 * | pH 9.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CHES | 0.05 (M) | |
| 2 | 1 | drop | protein | 16 (mg/ml) | |
| 3 | 1 | reservoir | sodium citrate | 1.20 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 200 (mM) | |
| 5 | 1 | reservoir | MPD | 5 (%) |
