1H72
CRYSTAL STRUCTURE OF HOMOSERINE KINASE COMPLEXED WITH HSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.990, 87.990, 99.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.750 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.800 | |
| Rmerge | 0.089 | |
| Total number of observations | 206493 * | |
| Number of reflections | 36728 | |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 99.8 | 99.9 * |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 100 * | PEG4000, SODIUM ACETATE, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protease | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | substrate | 5 (mM) | |
| 7 | 1 | reservoir | Tris | 0.1 (M) | |
| 8 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 9 | 1 | reservoir | PEG4000 | 20-25 (%) |
