1H65

Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	110
Detector technology	CCD
Collection date	1998-06-15
Detector	ADSC CCD
Wavelength(s)	0.9796, 0.9794, 0.9680, 0.9802
Spacegroup name	C 1 2 1
Unit cell lengths	143.160, 78.673, 67.279
Unit cell angles	90.00, 91.44, 90.00

Refinement procedure

Resolution	31.000 * - 2.000
R-factor	0.186
Rwork	0.186
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	21.900 *
Data reduction software	DPS
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.056	0.223
Number of reflections	50147
<I/σ(I)>	8	1.9
Completeness [%]	99.0	98.8
Redundancy	7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	THE TOC34 CRYSTALS WERE GROWN AT 4 DEGREES CELSIUS BY HANGING DROP VAPOR DIFFUSION. A 2-MICROLITER SOLUTION (10 MG/ML IN 50 MM TRIS-HCL AT PH 8.0 AND 0.1 M NACL) WAS MIXED WITH A 2-MICROLITER RESERVOIR SOLUTION CONTAINING 22% PEGMME 5K AND 10% GLYCEROL IN 0.1 M HEPES AT PH 6.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH8.0
3	1	drop		0.1 (M)
4	1	reservoir	PEG5000 MME	22 (%(w/v))
5	1	reservoir	glycerol	10 (%(v/v))
6	1	reservoir	HEPES	0.1 (M)	pH6.5