1H64
CRYSTAL STRUCTURE OF THE SM-RELATED PROTEIN OF P. ABYSSI: THE BIOLOGICAL UNIT IS A HEPTAMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.330, 70.160, 116.010 |
| Unit cell angles | 90.21, 97.70, 107.48 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODELLED HEPTAMER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 * |
| Data reduction software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.147 |
| Total number of observations | 386356 * | |
| Number of reflections | 156396 * | |
| <I/σ(I)> | 14 | 4 |
| Completeness [%] | 96.2 * | 95.2 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 22 * | MPD, MAGNESIUM ACETATE, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 27-29 (%) | |
| 2 | 1 | reservoir | magnesium acetate | 150 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
