1H5S
Thymidylyltransferase complexed with TMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Collection date | 2000-07-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.100, 119.270, 81.110 |
| Unit cell angles | 90.00, 112.66, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.300 |
| Rwork | 0.176 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.067 |
| Number of reflections | 56185 |
| <I/σ(I)> | 10.1 |
| Completeness [%] | 99.0 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.50 |
