1H4P
Crystal structure of exo-1,3-beta glucanse from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.597, 102.597, 203.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.750 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cz1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.000 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.043 | 0.443 * |
| Total number of observations | 107423 * | |
| Number of reflections | 104421 | |
| <I/σ(I)> | 37 | 2.8 |
| Completeness [%] | 97.6 * | 86.9 * |
| Redundancy | 12 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 18 * | 100 MM SODIUM HEPES PH 7.8, 25 MM MGCL2, 1.3 M TRI-SODIUM CITRATE, 20% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | trisodium citrate dihydrate | 1.2 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH8. |
| 4 | 1 | reservoir | 25 (mM) |
