1H4A

Human GammaD Crystallin R58H mutant structure AT 1.15 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	33.467, 53.234, 89.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.330 * - 1.150
R-factor	0.1576 *
Rwork	0.155
R-free	0.18220 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1elp
RMSD bond length	0.751 *
RMSD bond angle	1.453 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.330	1.210
High resolution limit [Å]	1.150	1.150
Rmerge	0.050	0.109
Total number of observations	463412 *
Number of reflections	55087
<I/σ(I)>	9.2	6.3
Completeness [%]	95.3	97.6
Redundancy	4.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	277	10MM PHOSPHATE, 4 DEGREE CELSIUS, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium phosphate	100 (mM)	pH7.
2	1	1	dithiothreitol	20 (ml)