1H3P

STRUCTURAL CHARACTERISATION OF A MONOCLONAL ANTIBODY SPECIFIC FOR THE PRES1 REGION OF THE HEPATITIS B VIRUS

Replaces:  1GM3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1998-08-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	47.010, 88.460, 111.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.2126
Rwork	0.213
R-free	0.29080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h1l
RMSD bond length	0.005
RMSD bond angle	29.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.581)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.077 *	0.272
Number of reflections	14823
<I/σ(I)>	9.8	5.6
Completeness [%]	98.3	88
Redundancy	6.5	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	17 *	0.1M CYSTEINE, 15% PEG6000, 110MM MGCL2, 60MM TRISHCL PH8.5, 15% GLYCEROL, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cysteine	0.1 (M)
2	1	reservoir	PEG6000	15 (%(w/v))
3	1	reservoir		110 (mM)
4	1	reservoir	Tris-HCl	60 (mM)	pH8.5
5	1	reservoir	glycerol	15 (%)
6	1	drop	protein	4.7 (mg/ml)