1H0J
Structural Basis of the Membrane-induced Cardiotoxin A3 Oligomerization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2001-09-15 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.902, 76.202, 47.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.27750 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tgx |
RMSD bond length | 0.005 |
RMSD bond angle | 25.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.046 | 0.266 |
Total number of observations | 93866 * | |
Number of reflections | 21036 | |
<I/σ(I)> | 28.5 | 3.91 |
Completeness [%] | 94.4 | 96.3 |
Redundancy | 4.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | 10 MG/ML PROTEIN MIXED WITH 0.1M SODIUM ACETATE (PH 4.6), 20% PEG 400, 3% GLYCEROL, AND 24 MM SDS. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
3 | 1 | reservoir | PEG400 | 20 (%) | |
4 | 1 | reservoir | glycerol | 3 (%) | |
5 | 1 | reservoir | SDS | 24 (mM) |