1H04
Human CD55 domains 3 & 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.700, 20.400, 52.700 |
| Unit cell angles | 90.00, 111.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h03 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | 0.245 |
| Total number of observations | 45372 * | |
| Number of reflections | 14289 | |
| <I/σ(I)> | 3.5 | 1.1 |
| Completeness [%] | 99.2 * | 92 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.6 * | Lea, S.M., (1999) Acta Crystallogr.,Sect.D, D55, 1198. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 30 (%) | |
| 2 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium acetate trihydrate | 0.1 (M) | pH4.6 |
| 4 | 1 | drop | protein | 8-12 (mg/ml) |
