1H04

Human CD55 domains 3 & 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	125.700, 20.400, 52.700
Unit cell angles	90.00, 111.50, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.214
Rwork	0.214
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h03
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.000	2.000
Rmerge	0.085	0.245
Total number of observations	45372 *
Number of reflections	14289
<I/σ(I)>	3.5	1.1
Completeness [%]	99.2 *	92
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.6 *		Lea, S.M., (1999) Acta Crystallogr.,Sect.D, D55, 1198. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30 (%)
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	sodium acetate trihydrate	0.1 (M)	pH4.6
4	1	drop	protein	8-12 (mg/ml)