1H03

Human CD55 domains 3 & 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-15
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	38.620, 38.770, 45.330
Unit cell angles	110.48, 96.55, 108.90

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.191
Rwork	0.191
R-free	0.24100 *
Structure solution method	MIRAS
RMSD bond length	0.014
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.062	0.350
Total number of observations	685390 *
Number of reflections	44162
<I/σ(I)>	10.4	1.5
Completeness [%]	100.0	92
Redundancy	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.6 *		Lea, S.M., (1999) Acta Crystallogr.,Sect.D, D55, 1198. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30 (%)
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	sodium acetate trihydrate	0.1 (M)	pH4.6
4	1	drop	protein	8-12 (mg/ml)