1GZY

Human Insulin-like growth factor; In-house data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	30.723, 69.282, 64.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 * - 2.540
R-factor	0.218
Rwork	0.213
R-free	0.27200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ins
RMSD bond length	0.013 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000 *	2.070
High resolution limit [Å]	2.540 *	2.540 *
Rmerge	0.086 *	0.331 *
Total number of observations	8654 *
Number of reflections	2065 *
<I/σ(I)>	18	12
Completeness [%]	98.1 *	95 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		THE PROTEIN WAS CRYSTALLIZED BY THE HANGING DROP METHOD IN WHICH DROPS WERE COMPOSED OF VARIOUS RATIOS OF HIGF-I AT 7MG/ML (IN H2O) WITH RESERVOIR SOLUTION CONSISTING OF 0.1M TRIS.HCL PH 7.5, 12-15% (W/V) PEG 2K AND 5MM SB12 DETERGENT.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)	in water
2	1	reservoir	Tris-HCl	0.1 (M)	pH7.5
3	1	reservoir	PEG2000MME	12-15 (%(w/v))
4	1	reservoir	SB12 detergent	5 (mM)