1GZT
Pseudomonas aeruginosa lectin II (PA-IIL) together with fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.660, 72.920, 54.560 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 30.020 - 1.300 |
| R-factor | 0.137 |
| Rwork | 0.136 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HO DERIVATIVE SOLVED WITH (REMOTE ENERGY) SAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.260 * |
| Data reduction software | HKL |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.049 | 0.301 |
| Total number of observations | 324838 * | |
| Number of reflections | 96602 | |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 95.8 * | 70.9 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 20 * | TRIS HCL 0.1M, PH8.5, 1.75 M AMMNONIUM SULFATE, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | fucose | 250 (ug/ml) | |
| 3 | 1 | drop | salts | 2 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.75 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
