1GZM
Structure of Bovine Rhodopsin in a Trigonal Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 103.820, 103.820, 76.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.650 |
| R-factor | 0.2015 |
| Rwork | 0.202 |
| R-free | 0.23500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f88 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.293 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.119 | 0.434 |
| Number of reflections | 26026 | |
| <I/σ(I)> | 11 | 1.4 |
| Completeness [%] | 97.0 | 86.1 * |
| Redundancy | 4.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 18 * | Edwards, P., (2004) J. Mol. Biol., 343, 1439. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | 0.6-1.1 (M) | ||
| 3 | 1 | reservoir | PEG8000 | 1.6 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%(v/v)) |
