1GZJ
Structure of Thermoascus aurantiacus family 5 endoglucanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.510, 85.850, 89.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.940 - 1.620 |
R-factor | 0.159 |
Rwork | 0.159 |
R-free | 0.17700 |
Structure solution method | MIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.250 * |
Data reduction software | DENZO |
Data scaling software | Agrovata |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.350 | 1.680 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.056 | 0.289 |
Number of reflections | 60922 | |
Completeness [%] | 81.6 | 83.9 |
Redundancy | 5.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 291 * | Loleggio, L., (1997) Acta Crystallogr.,Sect.D, 53, 599. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12.3 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
3 | 1 | reservoir | ammonium sulfate | 2.0 (M) |