1GZC
High-Resolution crystal structure of Erythrina cristagalli lectin in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.800, 81.800, 126.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 1.600 * |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ax1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 1.640 |
| High resolution limit [Å] | 1.600 * | 1.580 |
| Rmerge | 0.053 | 0.608 |
| Total number of observations | 551905 * | |
| Number of reflections | 58614 | 6065 * |
| <I/σ(I)> | 20.9 | 2.3 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 9.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lactose | 13 (%) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
