1GZ9

High-Resolution Crystal Structure of Erythrina cristagalli Lectin in Complex with 2'-alpha-L-Fucosyllactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-01-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	80.900, 80.900, 125.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.370 - 1.700
R-factor	0.209
Rwork	0.209
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ECL LACTOSE COMPLEX
RMSD bond length	0.014 *
RMSD bond angle	1.980 *
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.067	0.693 *
Total number of observations	429936 *
Number of reflections	46349	7167 *
<I/σ(I)>	17.5	2.6
Completeness [%]	99.3	99.2
Redundancy	9.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	lactose	13 (%)
2	1	drop	HEPES	20 (mM)
3	1	drop		100 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	ammonium sulfate	2 (M)
6	1	reservoir	Tris-HCl	0.1 (M)	pH8.0