1GZ0
23S RIBOSOMAL RNA G2251 2'O-METHYLTRANSFERASE RLMB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.478, 132.288, 90.710 |
| Unit cell angles | 90.00, 96.40, 90.00 |
Refinement procedure
| Resolution | 47.000 * - 2.500 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.061 * | 0.371 * |
| Total number of observations | 286761 * | |
| Number of reflections | 70653 * | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 21 * | PEG400, TRIS-HCL, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.5 |
| 3 | 1 | drop | 500 (mM) | ||
| 4 | 1 | drop | glycerol | 5 (%(w/v)) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | PEG400 | 15 (%(v/v)) | |
| 7 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.5 |
| 8 | 1 | reservoir | ammonium acetate | 440 (mM) |
