1GYY

The Crystal Structure of YdcE, a 4-Oxalocrotonate Tautomerase Homologue from Escherichia coli, Confirms the Structural Basis for Oligomer Diversity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-10-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	44.700, 46.700, 76.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.350
Rwork	0.151
R-free	0.17300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIALLY REFINED 1.5A RESOLUTION NATIVE
RMSD bond length	0.009 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.390
High resolution limit [Å]	1.350	1.350
Rmerge	0.037	0.159 *
Total number of observations	276503 *
Number of reflections	36652 *
<I/σ(I)>	29.5	4.2
Completeness [%]	91.4	35
Redundancy	7.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *	PROTEIN: 25MG/ML IN 50MM HEPES, PH 7.3, 4 CELCIUS, SITTING DROP: MIX 15UL PROTEIN WITH 5UL 40% SODIUM CITRATE PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium citrate	40 (%(w/v))	pH8.5