1GYT

E. coli Aminopeptidase A (PepA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-15
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	178.000, 178.000, 244.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 * - 2.500
R-factor	0.183 *
Rwork	0.165
R-free	0.21000 *
Structure solution method	MIR
RMSD bond length	0.005 *
RMSD bond angle	1.230 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.098 *	0.307
Total number of observations	1198951 *
Number of reflections	298951
<I/σ(I)>	11.5	3.9
Completeness [%]	99.9	99.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8		20 MM KCL, 50 MM TRIS PH 8.0, 1 MM MG(AC)2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	8 (mg/ml)
2	1	2		200 (mM)
3	1	2	Tris	50 (mM)	pH8.0
4	1	2		1 (mM)