1GYO
Crystal structure of the di-tetraheme cytochrome c3 from Desulfovibrio gigas at 1.2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 56.670, 56.670, 94.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.500 - 1.200 |
| R-factor | 0.124 * |
| R-free | 0.14800 * |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.440 | 1.210 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.097 | 0.437 |
| Number of reflections | 104411 | |
| <I/σ(I)> | 16.3 | 2.8 |
| Completeness [%] | 98.7 | 95.6 |
| Redundancy | 4.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SALTING OUT FROM A PROTEIN SOLUTION IN TRIS/MALEATE PH=6.5, pH 6.50 |
