1GYL

INVOLVEMENT OF TYR24 AND TRP108 IN SUBSTRATE BINDING AND SUBSTRATE SPECIFICITY OF GLYCOLATE OXIDASE

Experimental procedure

Spacegroup name	P 4 2 2
Unit cell lengths	145.500, 145.500, 100.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.500 * - 3.000
R-factor	0.254
Rwork	0.254
RMSD bond length	0.016
RMSD bond angle	2.590
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.600 *
High resolution limit [Å]	3.000 *	3.000 *
Rmerge	0.092 *
Total number of observations	41999 *
Number of reflections	18421
Completeness [%]	82.7	71.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium acetate	1 (M)
3	1	reservoir	octyl pyranoside	0.4 (%)
4	1	reservoir	imidazol	100 (mM)