1GYH
Structure of D158A Cellvibrio cellulosa alpha-L-arabinanase mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.556, 79.557, 132.402 |
| Unit cell angles | 88.83, 89.58, 83.58 |
Refinement procedure
| Resolution | 20.000 - 1.900 * |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.20000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAP FROM AN A P6522 CRYSTAL FORM SOLVED BY MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.269 |
| Number of reflections | 155379 | |
| <I/σ(I)> | 12.37 | 3.08 |
| Completeness [%] | 97.4 | 89.8 |
| Redundancy | 1.9 * | 1.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 18% PEG5000 MME, 4% AMMONIUM SULFATE, 100MM NA-CACODYLATE PH6.5, 20% GLYCEROL, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG5000 MME | 18 (%(w/v)) | |
| 2 | 1 | 1 | ammonium sulfate | 4 (%(w/v)) | |
| 3 | 1 | 1 | sodium cacodylate | 100 (mM) | pH6.5 |
| 4 | 1 | 1 | glycerol | 20 (%(v/v)) |
