1GYE

Structure of Cellvibrio cellulosa alpha-L-arabinanase complexed with Arabinohexaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	90.605, 90.605, 177.554
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.19
Rwork	0.188
R-free	0.24000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE STRUCTURE SOLVED PREVIOUSLY
RMSD bond length	0.019
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.082	0.447
Number of reflections	15610
<I/σ(I)>	30.1	6.1
Completeness [%]	100.0	100
Redundancy	10.5 *	10.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8		10% PEG8000, 100MM TRIS-HCL PH8.0, 20% GLYCEROL, 100MM ARABINOTRIOSE, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	10 (%(w/v))
2	1	1	Tris-HCl	100 (mM)	pH8.0
3	1	1	glycerol	20 (%(v/v))
4	1	1	protein	20 (mg/ml)