1GXM
Family 10 polysaccharide lyase from Cellvibrio cellulosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.670, 106.113, 55.365 |
| Unit cell angles | 90.00, 91.95, 90.00 |
Refinement procedure
| Resolution | 15.710 - 1.320 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.16200 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.365 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.058 | 0.329 |
| Number of reflections | 112216 | |
| <I/σ(I)> | 17.9 | 2.4 |
| Completeness [%] | 86.8 | 35.4 |
| Redundancy | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | Charnock, S.J., (2001) Acta Crystallogr., D57, 1141. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | sodium MES | pH5.2 | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | PEG2000 MME | 20 (%(w/v)) |
